Phospholipase D activity of gram-negative bacteria
Publication information:
Cole and Proulx. 1975. “Phospholipase D Activity of Gram-Negative Bacteria”. J Bacteriol, 124, 3, Pp. 1148-52. doi:10.1128/jb.124.3.1148-1152.1975
Abstract
A phospholipase hydrolyzing cardiolipin to phosphatidic acid and phosphatidyl glycerol was characterized in gram-negative bacteria but was absent in preparations of gram-positive bacteria, Saccharomyces cerevisiae, and rat liver mitochondria. In cell-free extracts of Escherichia coli, Salmonella typhimurium, Proteus vulgaris, and Pseudomonase aeruginosa, this cardiolipin-hydrolyzing enzyme had similar pH and Mg2+ requirements and displayed a specificity which excluded phosphatidyl glycerol and phosphatidyl ethanolamine as substrates.